Transformation of colonial Escherichia coli on solid media

نویسندگان

چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Colonial Growth of Anaerobic Spirochetes on Solid Media.

Hardy, Paul H., Jr. (The Johns Hopkins University School of Medicine, Baltimore, Md.), Young C. Lee, and E. Ellen Nell. Colonial growth of anaerobic spirochetes on solid media. J. Bacteriol. 86:616-626. 1963.-A total of 14 strains of treponemes and one of Borrelia vincentii were cultivated as colonies on agar plates inoculated under ordinary atmospheric conditions, but incubated anaerobically. ...

متن کامل

Colonial morphology of Escherichia coli on Tergitol-7 medium.

Escherichia coli cultures grown on Tergitol-7 medium, with 2,3,5-triphenyltetrazolium chloride added, produced three main types of colonies: rough, intermediate, and mucoid. These colonies were yellow to amber in color and produced slight yellow zones in the medium. Rough colonies were flat, dry, and spreading, with a cut-glass appearance. Intermediate-type colonies varied considerably, but cou...

متن کامل

Microwave improved Escherichia coli transformation.

AIMS The calcium chloride chemical transformation of Escherichia coli is still the most widly used cloning method in small laboratories. Therefore, any practicable improvement in its transformation efficiency seems to be of general interest. METHODS AND RESULTS We found that giving calcium chloride competent cells a 1 min microwave pulse at the lowest power setting (180 W), instead of the cla...

متن کامل

Natural plasmid transformation in Escherichia coli.

Although Escherichia coli does not have a natural transformation process, strains of E. coli can incorporate extracellular plasmids into cytoplasm 'naturally' at low frequencies. A standard method was developed in which stationary phase cells were concentrated, mixed with plasmids, and then plated on agar plates with nutrients which allowed cells to grow. Transformed cells could then be selecte...

متن کامل

Transformation in Escherichia coli: cryogenic preservation of competent cells.

Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 2004

ISSN: 0378-1097

DOI: 10.1016/j.femsle.2004.05.023